University of Central Florida Undergraduate Research Journal - Preventing Introductions to Sustain Healthy Ecosystems: Establish Eradication Protocols for a Popular Aquarium Seaweed
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Trial 1

Our results sought out 100% mortality as that is what the aquarium hobbyist industry requires for promotion. No control fragments in saltwater died during the 5–week trial (Table 4, Figure 1). 1% or higher acetic acid, however, caused 100% mortality of both 1 cm and 10 cm fragments after 5 weeks. The lowest dose of 0.05 acetic concentration caused no mortality. Further analysis revealed that a 4% × 30 minute and 2% × 60–minute dose × exposure treatments caused 100% mortality within 24 hours.

Trial 2

Trial 2 was run to replicate Trial 1 and ensure consistency of results. The primary difference between Trials 1 and 2 were that the former found mortality at 1% concentration at 5 weeks, while mortality at the same concentration/week combination did not occur in Trial 2 (Table 5, Figure 1). Thus, again, the 4% × 30 minute and the 2% × 60–minute dose × exposure treatments were effective at killing C. linum within 24 hours.

Trial 3

Trial 3 (Table 6) aimed to narrow the concentration range of acetic acid to determine the minimum effective dosage needed to cause 100% mortality in C. linum over the short term. The only tested treatment to cause 100% mortality of fragments at 24 hours was 4% acetic acid at 10–min exposure. The other treatment combinations did not cause full mortality, even after 72 hours.

Trial 4

Trial 4 was run to determine a practical way for casual hobbyist to apply acetic acid to rid unwanted C. linum from their tanks. All tested commercial vinegars (5–6% acetic acid) caused 100% mortality of all 10–cm long C. linum fragments within 24 hours when exposed for 10 minutes (Table 7, Figure 2).

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